通路抗体+分子量
Akt (pan) :兔 60kDa
E-cadherin:兔 125kDa
Foxa1: 兔 50kDa
GLI1:鼠 160kDa hedgehog
MMP2:兔 72kDa,62kDa pro&active
MMP9: 兔 67kDa,92kDa
N-Cadherin:兔 140kDa CST
N-Cadherin:兔 130kDa PROTECH
NF-κB p65:兔 65kDa
NF-κB1 p105/p50:兔 50,150kDa
p38 MAPK: 兔 40kDa
Phospho-Akt:兔 60kDa
Phospho-mTOR:兔 289kDa
Phospho-p38 MAPK: 兔 43kDa
Phospho-PI3 Kinase p85 (Tyr458)/p55 : 兔 60 kDa and 85 kDa
Phospho-SAPK/JNK:鼠 46 kDa,和54 kDa
Phospho-TAK1:兔 82kDa
PI3 Kinase p85:兔 85kDa
AntiPhosphoMEK1/2:兔 43kDa
TAK1:兔 78-82 kDa()
TIMP1: 兔 26 kDa(抑制MMP)
TIMP2: 兔 22 kDa
Vimentin: 兔 54 kDa
GAPDH: 36kDa
2022:
LIF: 鼠 Use a concentration of 3 - 5 µg/ml. Detects a band of approximately 22-34 kDa (predicted molecular weight: 22 kDa).
规格: 1 mg/ml--------1ug/ul
取3ml:需要9ug, 即9ul
(实际:LIF:3ml+15ul抗体 1:200??)
HOXA10: 羊 Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 42 kDa).
规格:50 µg 浓度为 0.5 mg/ml ------0.5ug/ul
取3ml:需要0.6ug, 即1.2ul
(实际:HOXA10: 2.2ul---4ml 1:500)
N-Cadherin:2ml+3ul,鼠
NID2 1:500, 3ML~6ul 兔
E-cadherin:兔 125kDa proteintech
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