单细胞简易分群-各种细胞marker基因

探序基因肿瘤研究院整理

T细胞:

CD3D,CD3E,CD4,CD8A,CD8B

还有注意γδT细胞

Treg:

CD4,FOXP3

B细胞:

CD79A,CD79B

DC细胞:

cDC1(human:XCR1+ CLEC9A+ CD141+):交叉递呈抗原给CD8+T细胞,

cDC2(CD11b+ SIRPα+ CD1c+):激活CD4+ T 细胞和CD8+T细胞。

浆细胞样DC (plasmacytoid DC,pDC)(CD123+ CLEC4C+.)

参考:

肿瘤浸润DC的表型和功能差异

人DC细胞marker

NK细胞:

CD56(NCAM1),CD16(FCGR3A)

人类NK细胞表面标志主要以CD16、CD56来认定。目前多以CD3-、CD16+、CD56+作为NK细胞典型标志。

FGFBP2, FCG3RA, CX3CR1(有些文献使用这些marker)

巨噬细胞:

CD68/CD163等。M1和M2型不太好分,更多marker可以参照赛默飞网站写的说明。https://www.thermofisher.cn/cn/zh/home/life-science/cell-analysis/cell-analysis-learning-center/immunology-at-work/macrophage-cell-overview.html

内皮细胞:

VWF,FCN3,DNASE1L3,

免疫细胞:

PRPTC

上皮细胞:

EPCAM

成纤维细胞:

FGF7, MME, DCN,LUM, GSN,DCN,ACTA2,FAP,PDGFRA

肌成纤维: MMP11、POSTN、CTHRC1、COL1A1、ACTA2 和 COL3A1(参考:https://www.cn-healthcare.com/articlewm/20230822/content-1595535.html

Fibroblasts were identified by their archetypal markers LUM, DCN, VIM, PDGFRA, and COL1A2 (参考:https://www.jianshu.com/p/5a1b1283b52e?utm_campaign=haruki)

项目文章 | 单细胞技术绘制结肠粘膜成纤维细胞图谱(参考:https://mp.weixin.qq.com/s?__biz=MzU5NjYzMzA4MA==&mid=2247534049&idx=1&sn=4bce0394aa9dcb0a0fe56b9d77a2cd37&chksm=fe5db176c92a3860b5987e49dfaf47981cdd49521aa529f31c33118f0c11fee6b626bd5e83f6&scene=27)

HSC(hematopoietic stem cells):

黑色素肿瘤细胞:

Tumor cells were identified using MLANA, MITF,and DCT. Tumor cells were further divided into subgroups by expression of PRAME and GEP genes("Single-cell analysis reveals new evolutionary complexity in uveal melanoma")

版权声明:
作者:玉兰
链接:https://www.techfm.club/p/111225.html
来源:TechFM
文章版权归作者所有,未经允许请勿转载。

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